Studies on the development of an Immunoassay to detect Neutrophil Collagenase (MMP-8)

by Suzanne C. Koston

Publisher: University College Dublin in Dublin

Written in English
Published: Pages: 98 Downloads: 710
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  • Metalloproteinases.,
  • Extracellular matrix -- Enzymology.,
  • Collagenases.,
  • Neutrophils.,
  • Enzyme-linked immunosorbent assay.

Edition Notes

Statementby Suzanne C. Koston.
ContributionsUniversity College Dublin. Department of Medicine and Therapeutics.
The Physical Object
Pagination(7), vii, 98p. :
Number of Pages98
ID Numbers
Open LibraryOL17401194M

  Neutrophils are an important part of the innate immune system and constantly enter the oral cavity 10 (Figs. 1 & 2).Neutrophils enter from the gingival sulcus, and come in contact with .   Project Summary:A current problem in developing new vaccines for Mycobacterium avium ss paratuberculosis infection in cattle is the difficulty in evaluating MAP vaccine efficacy in the bovine. . Matrix metallopeptidases (MMPs), also known as matrix metalloproteinases or matrixins, are metalloproteinases that are calcium-dependent zinc-containing endopeptidases; other family InterPro: IPR   The purpose of this study was to examine the role of interstitial collagenases, members of the family of matrix metalloproteinases, in the development of pulmonary fibrosis. The activity, Cited by:

Neutrophils alleviate fibrosis in the CCl 4 ‐induced mouse chronic liver injury model Eiko Saijou. Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo, Japan MMP8 is a known neutrophil collagenase, The most interesting finding of our study is that neutrophils Cited by:   Degradation of components of the extracellular matrix such as elastin and collagen by elastase and collagenase accelerates skin aging. Phytochemicals that inhibit the activity of these enzymes can be developed as anti-aging ingredients. In this study Cited by: 5. genase (MMP-1) (1), neutrophil collagenase (MMP-8) (2), and collagenase 3 (MMP) (3). These enzymes play a major role in connective tissue remodeling, in-cluding degradation of native type II collagen, the major structural protein of cartilage (4). A collagenase Cited by: Elevated neutrophil-to-lymphocyte ratio in patients with ovarian cancer has been found to be associated with poor prognosis (Zhou et al., ), but the significance of neutrophils in this disease has been questioned by a study Cited by:

Matrix metallopeptidase 8 (neutrophil collagenase), also known as MMP8, is a part of the MMP family which are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development. @article{osti_, title = {Granulocyte-associated IgG in neutropenic disorders}, author = {Cines, D B and Passero, F and Guerry, D and Bina, M and Dusak, B and Schreiber, A D}, abstractNote = {We applied a radiolabeled antiglobulin test to a study of patients with a variety of neutropenic disorders. After defining the nature of the interaction of radiolabeled anti-IgG with the neutrophil. Diabetic mice have impaired neutrophil phagocytosis of the periodontal pathogen Porphyromonas gingivalis, and clearance of the same pathogen was deficient, so more severe tissue damage was evident. 46 Adding RvE1 to the phagocytosis assay increased P. gingivalis phagocytosis and killing by neutrophils .

Studies on the development of an Immunoassay to detect Neutrophil Collagenase (MMP-8) by Suzanne C. Koston Download PDF EPUB FB2

Development of a novel immunoassay for the simple and fast quantitation of neutrophil gelatinase-associated lipocalin using europium(III) chelate microparticles and magnetic beads Author links open overlay panel Hao Wang a Xiangming Zhai a Tiancai Liu a Junyu Liang a Lun Bian a Li Lin a Zhenhua Chen a Peng Li a Zhining Dong b Zhixiong Li b Yingsong Wu aAuthor: Hao Wang, Xiangming Zhai, Tiancai Liu, Junyu Liang, Lun Bian, Li Lin, Zhenhua Chen, Peng Li, Zhining.

MMP-8, also known as neutrophil collagenase, is expressed not only by neutrophils, as it was suggested before, but also by macrophages.

Substrates for MMP-8 include both ECM (e.g., aggrecan and collagen) and non-ECM (e.g., angiotensin and plasminogen) proteins. Inflammation is an essential process in wound healing and for the elimination of invading pathogens. However, unregulated inflammation can lead to numerous pathologies including autoimmunities, Author: Jennifer H.

Cox. Results: Neutrophil collagenase-2 immunoassay was 96% sensitive for poor oral hygiene, 95% sensitive for chronic periodontitis (defined as at least two sites with periodontal pockets), and %. A new immunoassay was developed to detect denaturation of type II collagen in osteoarthritis (OA).

Ulcerative colitis, an inflammatory bowel disease, is associated with the massive infiltration of neutrophils. Although the initial infiltration of neutrophils is beneficial for killing bacteria, it is.

The Neutrophil Elastase Activity Assay Kit utilizes the ability of NE to proteolytically cleave a synthetic substrate and release a fluorophore, AFC, which can be easily quantified by fluorescence.

This assay kit is simple, rapid, and can detect as low as 1 ng of neutrophil elastase. The analysis of the disease-specific oral and systemic biomarkers in saliva and oral fluids (i.e., mouth rinse, gingival crevicular fluid (GCF), and peri-implantitis fluid (PISF)) is demanding.

Several hosts and Cited by: Increased type I collagen degradation products confirmed collagen turnover in AAAs (median values: mRNA and protein analysis identified neutrophil collagenase [matrix metalloproteinase (MMP)-8] and cysteine collagenases cathepsin K, L, and S as the principle collagenases Cited by:   Neutrophil elastase is a serine protease that has been implicated in the pathogenesis of inflammatory bowel disease.

Due to post-translational control of its activation and high expression of Cited by: 1. Neutrophil collagenase (matrix metalloproteinase 8 [MMP-8]) is an important mediator of tissue destruction in inflammatory diseases. Studies of anaerobic periodontal infections have shown that active MMP-8 in gingival crevicular fluid is associated with the degradation of periodontal tissues in progressive periodontitis whereas the latent enzyme is predominant in by: Neutrophils are white blood cells that play some very important roles in our innate immune system.

They circulate around our body in the bloodstream, and when they sense signals that an infection. complicated [1].

Neutrophil collagenase, also called matrix metalloproteinase (MMP)-8, polymorphonuclear (PMN) leukocyte collagenase, or collagenase-2, has been identified and Cited by: Research Article Inhibition of Neutrophil Collagenase/MMP-8 and Gelatinase B/MMP-9 and Protection against Endotoxin Shock ZhengQiu, 1 JianghaiChen, 2 HanmeiXu, 3 Steen, 4.

The role of tumor-associated neutrophils (TANs) in cancer progression versus regression remains controversial. TANs are known to have dichotomous antitumor (N1) and protumor (N2) phenotypes depending on the tumor microenvironment. Past studies Cited by:   Neutrophils mediate development of reparative macrophages.

a, b Mice were treated with anti-Ly6G mAb or IgG at 6 h after APAP challenge. The percentage and number of the indicated Cited by:   Neutrophils are classically considered as cells pivotal for the first line of defense against invading pathogens.

In recent years, evidence has accumulated that they are also important in the orchestration of adaptive immunity. Neutrophils Cited by: Start studying Microbiology Chapter 15 Book. Learn vocabulary, terms, and more with flashcards, games, and other study tools.

Studies on innate immunity have benefited from the introduction of zebrafish as a model system. Transgenic fish expressing fluorescent proteins in leukocyte populations allow direct, Cited by: Radioimmune Assay (RIA) Enyzme Linked Immune Sorbant Assay (ELISA) W es trnblo Immunoprecipitation Immunofluorescence Flow cytometry Expression cloning Radioimmunoassay (RIA) Cpm Amount unlabeled Antigen • Yalow and Berson (Nobel Prize) •Very sensitive: can detect.

Start studying Comprehensive Periodontics for the Dental Hygienist FINAL. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Cancer Immunology, Immunotherapy, ISSN8/, Vol Issue 8, pp.

-   Unlike the observations in our in vitro study, we saw that neutrophil trails were sparsely distributed and rather rarely shown in the in vivo imaging. We previously showed that microparticles generated from neutrophil trails had multiple spherical shapes ranging from to nm in diameter.

At present, it is not possible to detect Cited by:   Glucosamine is an amino-monosaccharide and precursor of glycosaminoglycans, major components of joint cartilage. Glucosamine has been clinically introduced for the treatment of osteoarthritis but the data about its protective role in disease are insufficient.

The goal of this study Cited by:   Neutrophil collagenase, also called matrix metalloproteinase (MMP)-8, polymorphonuclear (PMN) leukocyte collagenase, or collagenase-2, has been identified and. expression was coordinate with expression of macrophage elastase/MMP12 and neutrophil collagenase/MMP8 suggesting that macrophages and neutrophils were, at least in part, a source of cytokines and chemokines.

Toxicology and Applied Pharmacology () 52–59 Cytokines/chemokines multiplex immunoassay studiesFile Size: 2MB. Neutrophil disorders are an uncommon yet important cause of morbidity and mortality in infants and children. This article is an overview of these conditions, with emphasis on clinical Cited by: A set of 90 novel 2-(arylsulfonyl)-1,2,3,4-tetrahydroisoquinolinecarboxylates and -hydroxamates as inhibitors of the matrix metalloproteinase human neutrophil collagenase (MMP-8) was designed, synthesized, and investigated by 3D-QSAR techniques (CoMFA, CoMSIA) and X-ray structure analysis.

Docking studies Cited by:   In some cases, clonal hematopoiesis is found by the human androgen receptor assay (HUMARA) and in a few patients smaller (subdiagnostic) MDS-like clones are detected by FISH or by flow cytometry. 9–11,36,38,39 Currently, deep-sequencing studies and other studies Cited by: Neutrophils are the most abundant type of granulocytes and the most abundant type of white blood cells in most mammals.

They form an essential part of the innate immune system, with their functions varying in different animals. They are formed from stem cells in the bone marrow and differentiated into subpopulations of neutrophil-killers and neutrophil-cagers Function: Granulocyte. MMP8 ELISA Kit LS-F is a Well enzyme-linked immunosorbent assay for the Quantitative detection of Rat MMP8 in Plasma, Serum.

It is a Sandwich assay which can detect MMP8 down to .sensors Article Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase y Norlaily Ahmad 1,2, Burcu Colak 1, De-Wen Zhang 3, Martin John Cited by: 1.LS-F is a well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Guinea pig MMP8 in samples of Plasma and Serum.

It is based upon a Sandwich assay principle and can be used to detect levels of MMP8 as low as nanograms per millilter.